1. Trihydroxymethyl aminomethane rau kev coj ua lag luam synthesis

Vim nws cov qauv yooj yim, lub hauv paus carbon atom yog tetrahedral qauv, muaj peb hydroxymethyl thiab ib pawg amino, uas tuaj yeem ua ntau yam kev hloov pauv thiab REDOX cov tshuaj tiv thaiv, yog ib hom tshuaj lom neeg zoo synthesis raw khoom. Ib qho ntawm cov txheej txheem synthesis ntawm buffer TAPS tau ua los ntawm kev siv Tris thiab 1, 3-propanolone hauv cawv. Feem ntau, TRIS raw khoom siv rau kev lag luam synthesis tsis xav tau qhov purity siab ntawm cov reagent. Qhov tseem ceeb yog nyob rau ntawm ntau ntau, yog li nws yog qhov tsim nyog dua los nrhiav cov khoom siv raw khoom nrog lub peev xwm loj.

2. Trimethylol amino methane rau kev taw qhia biochemical nrhiav kom pom

Feem ntau Tris siv ua ib qho khoom siv los tswj cov pH ntawm cov tshuaj biochemical. Vim nws tsis muaj zog alkalinity, Tris feem ntau siv ua ke nrog cov kua qaub. Nws feem ntau yog siv rau nucleic acid, protein dissolution buffer, protein imprinting buffer, DNA imprinting experiment buffer, DNA agarose gel electrophoresis buffer, thiab lwm yam. Hauv cov tshuaj tiv thaiv vim muaj kev koom tes ntawm cov enzymes, cov protein thiab ntau yam tshuaj tua kab mob, yog li qhov yuav tsum tau muaj buffer siab heev, yog li kev yuav khoom yuav tsum xaiv lub chaw tsim khoom nrog ntau xyoo ntawm kev tshawb fawb thiab kev txhim kho thiab kev tsim khoom, kev ntshiab siab, tsawg dua impurities kom ntseeg tau tias qhov tseeb ntawm kev sim biochemical. Tris buffer kub nyhuv, yooj yim nqus cov pa roj carbon dioxide hauv huab cua, qhov kev teeb tsa ntawm buffer yuav tsum tau npog nruj nreem.

3. Trimethylol aminomethane rau kev tshawb fawb thiab kev txhim kho tshuaj

Peb lub hydroxymethyl aminomethane lwm lub npe hu ua trometamol, vim tsis muaj sodium amino buffer base, hauv cov kua dej hauv lub cev sib xyaw nrog H2CO3, txo H2CO3, tsim HCO32 - dua tib lub sijhawm, tuaj yeem nqus cov hydrogen ions thiab kho cov acidemia, nws cov nyhuv muaj zog dua, thiab tuaj yeem hla lub cell membrane, feem ntau siv rau hauv kev mob metabolic thiab respiratory acidemia; Ua ib qho tshuaj alkaline, siv rau kev kho acidosis yam tsis ua rau muaj cov pa roj carbon dioxide ntau ntxiv. Vim tias nws yog siv rau kev lag luam tshuaj, tag nrho cov khoom siv raw TRIS, txoj kev tsim khoom, cov khoom purity, cov ntsiab lus impurity ion yog cov kev cai nruj, kev yuav khoom ntawm cov khoom no yuav tsum nruj heev thiab ceev faj, yuav tsum dhau qhov kev xeem nruj.

4. Trimethylaminomethane siv rau hauv cov kua dej khaws cia kab mob

Hauv kev daws teeb meem tiv thaiv kab mob, Tris tuaj yeem tswj tau tus nqi pH ntawm cov qauv, ua haujlwm ua lub buffer biological, thiab pH buffer ntau ntawm Tris yog: 7.0 txog 9.0, lub peev xwm los tswj kom rho tawm cov qauv tawg tom qab tso tawm qhov ruaj khov ntawm nucleic acid, kom tsis txhob muaj kev puas tsuaj ntawm nucleic acid, thiab txhim kho qhov concentration thiab purity ntawm nucleic acid, lav qhov tseeb ntawm cov haujlwm tom ntej xws li kev tshuaj xyuas nucleic acid, vim yog lub sijhawm tshwj xeeb ntawm kev sib kis, kev xav tau kuaj nucleic acid, Tris yuav ntau dua, yog li xav kom yuav cov khoom zoo li no yuav tsum yuav me me ntawm cov khoom ua ntej yuav qhov kev sim loj tau lees paub tias tsis muaj teeb meem, kuj yuav tsum muaj kev txawj ntse tom qab muag los muab kev txhawb nqa kev txawj ntse.