Ethylenediaminetetraacetic acid (EDTA) yog ib yam khoom siv los ua kom cov hlau chelating ua ke. Qhov no hu ua chelating txhais tau tias nws tuaj yeem sib xyaw nrog qee cov hlau ions thiab ua rau cov hlau ions uas khi ua ke tsis ua haujlwm.

Siv cov yam ntxwv ntawm EDTA no, muaj ob hom kev siv hauv kev sim;

Ib hom yog daim ntawv thov rov qab, uas tiv thaiv qee yam kev tsis haum los ntawm kev cuam tshuam nrog kev khi ntawm cov hlau ions.

Siv ib qho ion: Hauv qee qhov kev tshuaj tiv thaiv, qhov kev sib txuas ntawm cov hlau ions yog qhov tseem ceeb. Peb muab EDTA nrog cov hlau ions no los tiv thaiv qhov kev tshuaj tiv thaiv no.

Piv txwv li, kev ntshav coagulation xav tau kev koom tes ntawm cov calcium ions. Peb ntxiv EDTA rau hauv cov ntshav kom chelate calcium ions nrog EDTA, yog li tiv thaiv cov ntshav coagulation. Qhov no yog lub hauv paus ntsiab lus ntawm EDTA anticoagulation;

Piv txwv li, kev npaj cov tshuaj decalcification hauv kev pleev xim rau cov kab mob kuj siv cov nyhuv chelation ntawm EDTA. Peb paub tias yog tias cov ntaub so ntswg uas muaj calcium ntau tsis raug decalcified, nws yuav nyuaj rau txiav ua cov ntaub so ntswg tiav thaum txiav, yog li peb yuav tsum tau decalcify ua ntej txiav.

Kuj tseem muaj ib hom kev thov rau pem hauv ntej uas txhawb nqa qee yam kev cuam tshuam los ntawm kev cuam tshuam nrog kev khi ntawm cov hlau ions.

Piv txwv li, ntxiv EDTA rau hauv cov kua zom trypsin. Yog tias tsis ntxiv EDTA, trypsin yuav khi rau calcium thiab magnesium ntawm qhov chaw ntawm lub cell lossis hauv cov kab lis kev cai, yog li ntawd txwv tsis pub trypsin ua haujlwm.

Lub sijhawm no, ntxiv me ntsis EDTA kom EDTA sib xyaw nrog cov calcium, magnesium thiab lwm cov ions divalent. Lub sijhawm no, ib qho xwm txheej zoo tau tsim rau qhov tshwm sim ntawm trypsin.

Qhov zoo kawg nkaus txog EDTA yog nws cov solubility. Nws yog me ntsis soluble hauv dej thiab zoo li dawb hauv dej. Lub sijhawm no, ntxiv sodium hydroxide los kho nws tus nqi pH. Thaum pH siab dua thiab siab dua, cov tshuaj yuav dhau los ua nws pom tseeb dua thiab meej dua. Thaum pH ze rau 8, nws yuav dhau los ua kom pom tseeb tag nrho. Nws tsis ntshai kua qaub tab sis alkali.

EDTA muaj ntau yam zoo kawg nkaus siv. Pom tau tias cov tshuaj hauv xwm muaj khawv koob heev, tab sis peb tsis tau pom nws.

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