Ethylenediaminetetraacetic acid (EDTA) yog cov hlau chelates. Lub npe hu ua chelating txhais tau hais tias nws tuaj yeem ua ke nrog qee cov hlau ions thiab ua rau cov hlau ions tsis ua haujlwm.

Siv cov yam ntxwv ntawm EDTA no, muaj ob hom kev siv hauv kev sim;

Ib hom yog daim ntawv thov rov qab, uas inhibits qee qhov kev cuam tshuam los ntawm kev cuam tshuam nrog kev khi ntawm cov hlau ions.

Siv ib qho ion: Hauv qee qhov kev cuam tshuam, kev khi ntawm cov hlau ions tseem ceeb. Peb muab EDTA nrog cov hlau ions los tiv thaiv cov tshuaj tiv thaiv no.

Piv txwv li, ntshav coagulation yuav tsum muaj kev koom tes ntawm calcium ions. Peb ntxiv EDTA rau cov ntshav kom chelate calcium ions nrog EDTA, yog li tiv thaiv cov ntshav coagulation. Qhov no yog lub hauv paus ntsiab lus ntawm EDTA anticoagulation;

Piv txwv li, kev npaj decalcification tov nyob rau hauv pathological staining kuj siv cov nyhuv chelation ntawm EDTA. Peb paub tias yog tias cov ntaub so ntswg uas tsis muaj calcium tsis yog decalcified, nws yuav nyuaj rau txiav rau hauv cov ntaub so ntswg kom tiav thaum txiav, yog li peb yuav tsum tau decalcify ua ntej slicing.

Kuj tseem muaj ib hom kev thov rau pem hauv ntej uas txhawb qee qhov kev cuam tshuam los ntawm kev cuam tshuam nrog kev khi ntawm cov hlau ions.

Piv txwv li, ntxiv EDTA rau trypsin digestion tov. Yog tias EDTA tsis ntxiv, trypsin yuav khi rau calcium thiab magnesium ntawm lub cell nto los yog hauv kab lis kev cai nruab nrab, yog li inhibiting trypsin kev ua.

Lub sijhawm no, ntxiv me ntsis EDTA kom tso cai rau EDTA ua ke nrog cov calcium dawb, magnesium thiab lwm yam divalent ions. Lub sijhawm no, qhov xwm txheej zoo yog tsim rau qhov tshwm sim ntawm trypsin.

Qhov zoo tshaj plaws ntawm EDTA yog nws cov solubility. Nws yog me ntsis soluble hauv dej thiab zoo li dawb hauv dej. Lub sijhawm no, ntxiv sodium hydroxide los kho nws tus nqi pH. Raws li pH tau siab dua thiab siab dua, cov tshuaj yuav ua kom pom tseeb thiab pom tseeb. Thaum pH ze rau 8, nws ua kom meej meej. Nws tsis ntshai acid tab sis alkali.

EDTA muaj ntau yam zoo siv. Nws tuaj yeem pom tau tias cov tshuaj nyob rau hauv qhov xwm txheej yog cov khawv koob heev, tab sis peb tsis tau pom nws.

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